10x Protocols: Visium Fresh Frozen Methanol Fixation, H&E Staining & Imaging-- University of Minnesota TMCs (CG000160 Rev C)
Laura J Niedernhofer, David A Bernlohr, 10X Genomics
Abstract
Protocols from 10x Genomics for Visium Spatial Gene Expression on Fresh Frozen / OCT samples. Completed without CytAssist component.
Version of protocol used:
10x protocol CG000160 Revision C -- Methanol Fixation, H&E staining, and imaging
Additional protocols added for guidance:
10x protocol for imaging guidelines (CG000241, Rev D)
10x protocol for tissue preparation guidelines (CG000240, Rev D)
Note: These protocols may not be the current version offered by 10x Genomics but were used to produce the specific datasets connected to them. Please review the 10x Genomics support website for the most recent versions of the protocols prior to starting your experiment.
Steps
10x protocol CG000160, Rev C (Fixation/H&E etc):
10x FF Visium Methanol Fixation and HE Staining.pdf
Additional Protocols/Resources
10x FF Visium Imaging Guidelines .pdf CG000241, Rev D
10x FF Visium Spatial Tissue Preparation Guide.pdf CG000240, Rev D
http://www.10xgenomics.com/support/spatial-gene-expression-fresh-frozen
