Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

Julia Y. Co, Mar Margalef-Català, Denise M. Monack, Manuel R. Amieva

Published: 2021-10-17 DOI: 10.1038/s41596-021-00607-0

organoids

epithelial polarity

host-microbe interactions

gastrointestinal health

disease research

AI 解读

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Human epithelial organoids—3D spheroids derived from adult tissue stem cells—enable investigation of epithelial physiology and disease and host interactions with microorganisms, viruses and bioactive molecules. One challenge in using organoids is the diff
Introduction
Materials
Procedure
- Apical-out GI epithelial organoids in suspension culture
- Downstream assays
Troubleshooting
Timing
Anticipated results
- References
References
ACKNOWLEDGMENTS

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Controlling the polarity of human gastrointestinal organoids to investigate epithelial biology and infectious diseases

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