E. coli Culture
Bailey Clark
Published: 2022-06-28 DOI: 10.17504/protocols.io.5jyl899o7v2w/v1
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Abstract
The abstract will be added later.
Before start
A video if unfamiliar: http://youtu.be/TWt62pgAEh0
Steps
Medium and Antibiotic Preparation
1.
Add 2mL (3mL also acceptable) of LB medium and the appropriate antibiotic that selects for the target strain into a 15 mL tube.
Kanamycin 50 mg/mL Stock Solution
2.
Place the 15mL tube in the ThermoMixer at 37°C, 300 rpm, for 0h 5m 0s .
Equipment
| Value | Label |
|---|---|
| ThermoMixer® C | NAME |
| Eppendorf | BRAND |
| Catalog No. 2231000680 | SKU |
Retrieving Cells
3.
Gather cells.
3.1.
Swab a single colony on the agar plate with a sterile 1µL inoculation loop.
3.2.
OR Dip a sterile 10 µL innoculation loop in a preexisting culture tube.
Innnoculation
4.
Innoculate the 15 mL tube with the loop.
5.
Place the 15 mL tube in the thermomixer for 16 hours at 37°C and 300 rpm.
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