Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

Yirong Chen, Meihua Lin, Dekai Ye, Shaopeng Wang, Xiaolei Zuo, Min Li

Published: 2024-02-05 DOI: 10.1038/s41596-023-00943-3

Circulating tumor cells

DNA frameworks

Aptamers

Targeted capture

Tetrahedral probes

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–7

Reporting Summary

Supplementary Data 5

Unprocessed gels for Supplementary Fig. 5

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

查看全部

Sections

Figures

References

Supplementary information

Alix-Panabières, C. & Pantel, K. Circulating tumor cells: liquid biopsy of cancer. Clin. Chem. 59, 110–118 (2013).
Ma, N. & Jeffrey, S. S. Deciphering cancer clues from blood. Science 367, 1424–1425 (2020).
Kelley, S. O. & Pantel, K. A new era in liquid biopsy: from genotype to phenotype. Clin. Chem. 66, 89–96 (2020).
Ozkumur, E. et al. Inertial focusing for tumor antigen–dependent and –independent sorting of rare circulating tumor cells. Sci. Transl. Med. 5, 179ra147 (2013).
Marquette, C. H. et al. Circulating tumour cells as a potential biomarker for lung cancer screening: a prospective cohort study. Lancet Respir. Med. 8, 709–716 (2020).
Steeg, P. S. Tumor metastasis: mechanistic insights and clinical challenges. Nat. Med. 12, 895–904 (2006).
Qin, W. et al. Bioinspired DNA nanointerface with anisotropic aptamers for accurate capture of circulating tumor cells. Adv. Sci. (Weinh.) 7, 2000647 (2020).
Song, Y. et al. Bioinspired engineering of a multivalent aptamer-functionalized nanointerface to enhance the capture and release of circulating tumor cells. Angew. Chem. Int. Ed. Engl. 58, 2236–2240 (2019).
Englund, E. A. et al. Programmable multivalent display of receptor ligands using peptide nucleic acid nanoscaffolds. Nat. Commun. 3, 614 (2012).
Zhao, W. et al. Bioinspired multivalent DNA network for capture and release of cells. Proc. Natl Acad. Sci. USA 109, 19626–19631 (2012).
Sheng, W., Chen, T., Tan, W. & Fan, Z. H. Multivalent DNA nanospheres for enhanced capture of cancer cells in microfluidic devices. ACS Nano 7, 7067–7076 (2013).
Yoon, H. J. et al. Sensitive capture of circulating tumour cells by functionalized graphene oxide nanosheets. Nat. Nanotechnol. 8, 735–741 (2013).
Shen, Z., Wu, A. & Chen, X. Current detection technologies for circulating tumor cells. Chem. Soc. Rev. 46, 2038–2056 (2017).
Gomes de Castro, M. A. et al. Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane. Nat. Commun. 10, 820 (2019).
Huppa, J. B. et al. TCR–peptide–MHC interactions in situ show accelerated kinetics and increased affinity. Nature 463, 963–967 (2010).
Seeman, N. C. & Sleiman, H. F. DNA nanotechnology. Nat. Rev. Mater. 3, 17068 (2017).
Rothemund, P. W. K. Folding DNA to create nanoscale shapes and patterns. Nature 440, 297–302 (2006).
Li, M. et al. Nucleic acid tests for clinical translation. Chem. Rev. 121, 10469–10558 (2021).
Lin, M. et al. Programmable engineering of a biosensing interface with tetrahedral DNA nanostructures for ultrasensitive DNA detection. Angew. Chem. Int. Ed. Engl. 54, 2151–2155 (2015).
Liu, Q. et al. Valency-controlled framework nucleic acid signal amplifiers. Angew. Chem. Int. Ed. Engl. 57, 7131–7135 (2018).
Yin, F. et al. DNA-framework-based multidimensional molecular classifiers for cancer diagnosis. Nat. Nanotechnol. 18, 677–686 (2023).
Ye, D., Zuo, X. & Fan, C. DNA nanotechnology-enabled interfacial engineering for biosensor development. Annu. Rev. Anal. Chem. 11, 171–195 (2018).
Zhao, S. et al. A DNA origami-based aptamer nanoarray for potent and reversible anticoagulation in hemodialysis. Nat. Commun. 12, 358 (2021).
Wilner, O. I. & Willner, I. Functionalized DNA nanostructures. Chem. Rev. 112, 2528–2556 (2012).
Chandrasekaran, A. R., Anderson, N., Kizer, M., Halvorsen, K. & Wang, X. Beyond the fold: emerging biological applications of DNA origami. Chembiochem 17, 1081–1089 (2016).
Kacherovsky, N. et al. Traceless aptamer-mediated isolation of CD8+ T cells for chimeric antigen receptor T-cell therapy. Nat. Biomed. Eng. 3, 783–795 (2019).
Mao, M. et al. Modular DNA-origami-based nanoarrays enhance cell binding affinity through the “lock-and-key” interaction. J. Am. Chem. Soc. 145, 5447–5455 (2023).
Lin, M. et al. Electrochemical detection of nucleic acids, proteins, small molecules and cells using a DNA-nanostructure-based universal biosensing platform. Nat. Protoc. 11, 1244–1263 (2016).
Li, M. et al. DNA framework-programmed cell capture via topology-engineered receptor–ligand interactions. J. Am. Chem. Soc. 141, 18910–18915 (2019).
Zhang, J. L. et al. DNA nanolithography enables a highly ordered recognition interface in a microfluidic chip for the efficient capture and release of circulating tumor cells. Angew. Chem. Int. Ed. Engl. 59, 14115–14119 (2020).
Chen, J. et al. Biospecific monolayer coating for multivalent capture of circulating tumor cells with high sensitivity. Adv. Funct. Mater. 29, 1808961 (2019).
Mitchell, N. et al. A DNA nanostructure for the functional assembly of chemical groups with tunable stoichiometry and defined nanoscale geometry. Angew. Chem. Int. Ed. Engl. 48, 525–527 (2008).

Functionalized tetrahedral DNA frameworks for the capture of circulating tumor cells

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Data 5

推荐阅读