GI Transit Assay
Sabina Marciano, Roberta Marongiu
Published: 2023-10-13 DOI: 10.17504/protocols.io.eq2lyjj3mlx9/v1
AI 解读 浏览 1400
Abstract
This test is used to check for possible signs of constipation and alteration in the gut transit
Steps
1.
Fast mice for 6 hours
2.
Administer 0.3 mL Carmine red per mouse via oral gavage
3.
Monitor the mice every 15 minutes for up to 5 hours for the appearance of the first red fecal pellet
4.
The time from gavage to passage of first red pellet is recorded as total intestinal transit time.
推荐阅读
Nature Protocols
Protocols IO
Current Protocols
