Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

Giulia Campostrini, Viviana Meraviglia, Elisa Giacomelli, Ruben W. J. van Helden, Loukia Yiangou, Richard P. Davis, Milena Bellin, Valeria V. Orlova, Christine L. Mummery

Published: 2021-03-25 DOI: 10.1038/s41596-021-00497-2

isogenic microtissues

human pluripotent stem cells

cardiac differentiation

3D tissue models

disease modeling

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–3.

Reporting Summary

Supplementary Table 1

Raw data for results summarized in Table 3. Table contains morphological, contraction and single-cell electrophysiological parameters measured in LUMC0099iCTRL04 and LUMC0020iCTRL06 hiPSC lines.

Supplementary Video 1

Beating monolayer hiPSC-CMs after 21 d of differentiation using LI-BPEL protocol with lactate purification

Supplementary Video 2

Beating monolayer hiPSC-CMs after 21 d of differentiation using mBPEL protocol

Supplementary Video 3

Time-lapse movie of the first 72 h of MT formation (time frame: 15 min)

Supplementary Video 4

Movie of representative MT showing CD31 positive ECs organized in a vessel-like structure (in red) and CMs stained with ACTN2 (in green) (15 f.p.s.)

Supplementary Video 5

Contracting MT paced at 1 Hz at after 21 d in culture

Supplementary Video 6

Contracting MT loaded with Fluo-4, paced at 1.5 Hz after 21 d in culture

References

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