Overview of scRNA-seq of Human Knee Articular Cartilage
molmer , Martin Lotz
Published: 2022-04-30 DOI: 10.17504/protocols.io.5qpvob93dl4o/v1
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Abstract
The overview provides sub-protocols for each step required to complete scRNA-seq from human knee articular cartilage.
Steps
1.
Tissue Acquisition
dx.doi.org/10.17504/protocols.io.14egn7996v5d/v1
2.
Tissue Quality Control
dx.doi.org/10.17504/protocols.io.n2bvj695nlk5/v1
3.
Single-Cell Isolation
dx.doi.org/10.17504/protocols.io.14egn765qv5d/v1
4.
cDNA Library Preparation
dx.doi.org/10.17504/protocols.io.bp2l6175rvqe/v1
5.
Data Processing and Quality Control
dx.doi.org/10.17504/protocols.io.j8nlkk3q6l5r/v1
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