Preparation of PEI for primary coating of MaxOne (neuronal cultures)
Yonatan Katz
Published: 2024-06-26 DOI: 10.17504/protocols.io.j8nlk8dy6l5r/v1
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Abstract
Polyethyleneimine (PEI) coating to enhance cell adhesion on MaxOne chips. PEI coating is followed by Laminin coating.
Steps
Materials
1.
0.07% PEI solution preparation - Primary coating
2.
Prepare 100 mL of 1X borate buffer:
5mL
95mL
3.
7% PEI stock solution :
1.4mL
8.6mL
Mix 0h 30m 0s using a magnetic stirrer until fully dissolved
(can be stored in 0.5 mL aliquots at -20°C for 1 month).
Where is it sterilized? -
4.
final 0.07% PEI solution
0.5mL in
49.5mL
filter through a 0.22 μm filter unit before use!
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